【摘要】 目的探讨白细胞介素10(IL10)在大鼠肝损伤过程中对肝星状细胞(HSC)表达转化生长因子β1(TGFβ1)和血小板源生长因子(PDGF)的影响。方法治疗组用含有IL10基因的腺病毒载体通过尾静脉转染大鼠(n=11),同时设立空载体组(n=12)和空白对照组(n=12),另给以皮下注射四氯化碳,每周2次,8周后处死大鼠,离体肝脏用胶原酶灌注以及密度梯度离心方法分离HSC。应用酶联免疫吸附法测大鼠血清中IL10水平,逆转录聚合酶链反应和蛋白印迹检测HSC的TGFβ1和PDGF表达。结果治疗组的IL10水平明显高于空载体组和空白对照组(P<005);治疗组HSC的TGFβ1和PDGF的mRNA、蛋白表达水平均低于空载体组和空白对照组(P<001)。结论IL10可以通过下调HSC的TGFβ1和PDGF的表达来减轻肝损伤时HSC的激活程度。更多还原

【Abstract】 Objective To investigate the effect of interlukin-10（IL-10）on the expression of transforming growth factor-β₁ （TGFβ₁） and platelet-derived growth factor （PDGF） in hepatic stellate cells （HSC） during liver injury. Methods The adenovirus vector （the titer was 1×107 efu/ml） encoded IL-10 gene was used to transfect the rat via the vein of caudal.At the same time, CCl₄ was injected into rat by a hypodermic injection.These processes went on twice a week. After eight weeks, the liver were perfused with collagenase Ⅳ and purified by density gradient centrifugation with Nycodenz for separate HSC. The level of IL-10 was measured by ELISA method; The expression of PDGF and TGFβ₁ in HSC was detected by semi-quantitative RT-PCR and Western-blot methods. Results The level of IL-10 in therapy group（adenovirus vector encoding IL-10 gene group） was higher than that in non-therapy group （adenovirus vector without IL-10 gene and PBS group）;The expression of TGFβ₁ mRNA, TGFβ₁ protein and PDGF mRNA, PDGF protein in therapy group were significantly lower than that in non-therapy group（P<0.05）. Conclusion^Downregulating the TGFβ₁ and PDGF expression could be the passageway by which IL-10 alleviate the degree of proliferation and activation in hepatic stellate cells.更多还原