【摘要】 目的探讨肤疾骨宁膏联合表皮生长因子(EGF)促进骨外露创面的愈合作用。方法将45只家兔随机分为3组:(1)肤疾骨宁膏联合EGF组;(2)肤疾骨宁膏组;(3)生理盐水组。实验60d,观察创面愈合情况及应用免疫组织化学和原位杂交技术检测EGF/EGFR及其mRNA的表达。结果肤疾骨宁膏联合EGF组创面愈合最快,生理盐水组创面愈合最慢(P<0.01)。创面在应用肤疾骨宁膏后,EGF/EGFR及其mRNA表达量逐渐增高,EGF及其mRNA表达量在15d达到高峰,EGFR及其mRNA表达量高峰期为15～22d,较生理盐水组明显增高,并且高峰提前(P<0.01)。结论肤疾骨宁膏联合EGF可有效促进骨外露创面的愈合。更多还原

【Abstract】 Objective To investigate the effect of Fujiguning ointment and epidermal growth factor (EGF) on the healing of wounds with bone exposed.Methods Forty-five rabbits were divided into 3 groups:Fujiguning ointment-EGF group,Fujiguning ointment group and normal saline group.During the experimental period of 60 days,the healing of wounds was observed and immunohistochemistry and in situ hybridization were employed to detect the expression of EGF/EGFR and EGF/EGFR mRNA in the granulation tissues.[WT5”HZ]Results The healing time of wounds with bone exposed was shorten obviously in Fujiguning ointment-EGF group as compared with Fujiguning ointment group and normal saline group (P< 0.01).EGF/EGFR was increased significantly,the expression of EGF and EGF mRNA reached the peak at the 15th day,and the expression of EGFR and EGFR mRNA reached the peak during 15th and 22th day in the Fujiguning ointment-EGF group and Fujiguning ointment group in comparison with normal saline group.Conclusion Fujiguning ointment and EGF can promote the healing of the wounds with bone exposed.更多还原