【摘要】 目的研究纤维蛋白原(Fg)Bβ启动子区-455G/A、-854G/A、-1420G/A3个单核苷酸多态性(SNP)与缺血性脑卒中(IS)的关系。方法应用病例对照分析和聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对160例IS患者与130名健康对照组的FgBβ-455G/A、-854G/A、-1420G/A等位基因频率和基因型频率与IS之间的关系进行分析。结果IS组的FgBβ-455G/A SNP A位点的等位基因频率为0·3688,与对照组的A位点的等位基因频率0·2538差异有统计学意义(P<0·01),IS组-455位点的基因型频率(GG:33·8%,GA:58·8%,AA:7·5%)与对照组的基因型频率(GG:55·4%,GA:38·5%,AA:6·2%)差异有统计学意义(P=0·01),IS组中-455位点的GA+AA基因型频率(66·3%)高于对照组的GA+AA频率(44·6%,P=0·001);在高血压分层分析后-455位点的A等位基因频率和GA+AA基因型频率在病例组和对照组之间差异有统计学意义,在两组间-854G/A、-1420G/A的基因型和等位基因频率的差异无统计学意义。结论FgBβ-455G/A位点与IS有关联,可能是IS的独立危险因素,-854G/A、-1420G/A与IS无关联。更多还原

【Abstract】 Objective To study the relationship between the single nucleotide polymorphisms(SNP) -455G/A、-854G/A、-1420G/A in B beta fibrinogen gene and ischemic stroke (IS) . Methods(Case-control) study was carried out to investigate allelic genotypes and frequency of the three B β fibrinogen (FgBβ) SNP in 160 subjects with IS and 130 control individuals. Polymerase chain reaction (PCR) and DNA sequencing were used to analyze the polymorphisms. The correlations between FgBβ SNPs and IS were evaluated. Results There was a highly significant difference in allele frequency of -455 G/A SNP between IS and control subjects, and the A allele frequencies were respectively 0.3688 versus 0.2538 (P<0.01). The frequencies of -455G/A SNP genotypes were significantly different (P=0.01) between the IS group (GG: 33.8%, GA:58.8%, AA:7.5%) and the control group (GG:55.4%, GA:38.5%, AA:6.2%). The frequency (66.3%) of genotype (GA+AA) of -455 G/A SNP in IS group were significantly higher than that (44.6% ) in the control group (P=0.001). The frequencies of allele A and genotype (GA+AA) of FgBβ-455 SNP were still associated with IS after stratification of blood pressure. No difference was found in the genotype and allelic frequencies of FgBβ-854G/A and -1420G/A alleles between IS group and the control groups. Conclusion Our results demonstrated that FgBβ-455G/A might be associated with IS and be an independent risk factor of IS. But we did not find that FgBβ-854G/A, -1420G/A SNP associated with IS.更多还原