【摘要】 目的探讨磷酸化信号传导和转录激活因子3(p-stat3)和E钙黏蛋白在表皮肿瘤的表达和意义。方法免疫组化方法观察30例皮肤鳞状细胞癌(SCC)、20例基底细胞上皮瘤(BCC)、20例脂溢性角化病(SK)肿瘤细胞中p鄄stat3和E钙黏蛋白的表达,20例正常人皮肤为对照。结果①p鄄stat3在皮肤SCC、BCC中呈明显的上调表达,皮肤SCC中p鄄stat3的表达强度明显高于BCC(P<0.05);②p鄄stat3在皮肤SCC中的表达强度与肿瘤的分化程度有关(P<0.05),阳性表达率与肿瘤浸润的深度有关(P<0.05),与肿瘤的大小无关。③皮肤SCC中E钙黏蛋白的表达明显减弱(P<0.001),且皮肤SCC中E钙黏蛋白的表达强度比皮肤BCC也明显减弱(P<0.05)。在皮肤SCC中E钙黏蛋白的表达强度与肿瘤的分化程度有关,阳性表达率与肿瘤浸润的深度和肿瘤的大小无关。④皮肤SCC中,p鄄stat3和E钙黏蛋白的阳性表达强度具有负相关性,rs=-0.37,P<0.05。结论p鄄stat3的表达异常可能在表皮肿瘤的发生中起重要作用。stat3的过度活化可能与皮肤SCC的侵袭性潜能密切相关。更多还原

【Abstract】 Objective To investigate stat 3 phosphorylation and E-cadherin expression and their clinical significance in human epidermal non-melanoma cutaneous tumors. Methods Immunohistochemistry technique was applied to measure the expression of p-stat 3 and E-cadherin protein in 30 cases of skin squamous cell carcinomas (SCC), 20 cases of basal cell carcinomas (BCC), 20 cases of seborrhoeic keratosis (SK) and 20 cases of normal subjects. Results ① p-stat 3 protein was significantly increased in SCC and BCC (P < 0.001), and the expression of stat 3 in SCC was significantly higher than that in BCC (P < 0.05). ② p-stat 3 expression in poorly differentiated SCC was higher than that in well differentiated SCC (P < 0.05). The positive rate of p-stat 3 expression was correlated with the depth of tumor invasion (P < 0.05), whereas there was no correlation between the positive rate and tumor size. ③ E-cadherin expression was significantly deceased in SCC (P < 0.001), and E-cadherin expression was lower in SCC than that in BCC (P < 0.05). In SCC, the intensity of E-cadherin expression was correlated with the extent of tumor differentiation, while there was no correlation between the expression and the depth of tumor invasion and the tumor size. ④There was a negative correlation between the expression intensity of p-stat 3 and E-cadherin in SCC (rs = -0.372, P < 0.05). Conclusions The overexpression of p-stat 3 may play an important role in the development of epidermal tumors. The abnormal activation of stat 3 may be related to the potentials of tumor invasion in SCC.更多还原