【摘要】 目的观察正常真皮内CD68阳性单核-巨噬细胞的分布、形态和密度。方法正常成人8例,每例均取面部、躯干、四肢近端、四肢远端、手掌和足跖6个部位皮肤进行表皮铺片和纵行与水平连续切片。CD68单克隆抗体染色,观察单核-巨噬细胞的分布。结果真皮浅层CD68阳性细胞可表现为树枝状及非树枝状,呈网状分布。真皮浅层CD68阳性细胞密度为:四肢远端(562±230)个/mm2,腹部(517±162)个/mm2,面部(509±235)个/mm2,手掌(507±192)个/mm2,四肢近端(472±138)个/mm2,足跖(361±78)个/mm2。真皮深层CD68阳性细胞密度低,多为树枝状,散在于胶原纤维间。结论CD68阳性单核-巨噬细胞在真皮浅层形成较致密网状分布。提示单核-巨噬细胞在真皮内有明确的方向性,其防御的方向是穿透表皮进入真皮的入侵物。更多还原

【Abstract】 Objective To study the distribution, morphology and density of CD68+ monocytes/macro,phages in normal human dermis. Methods Normal skin specimens from 6 sites including face, trunk, proximal limbs, distal limbs, palms and soles of 8 adults were collected. The epidermal sheets, horizontal and longitudinal sections of the specimens were stained with an ABC immunoperoxidase procedure with anti-CD68 monoclonal antibodies. Results The CD68+ cells were detected within the superficial dermis with various densities: 562 ± 230/mm2 at distal limbs, 517 ± 162/mm2 at trunk, 509 ± 235/mm2 at face, 507 ± 192/mm2 at palms, 472 ± 138/mm2 at proximal limbs and 361 ± 78/mm2 at soles. Lower densities of CD68+ cells with dendritic morphology were found in the deep (reticular) dermis, and dispersed among collagen bundles. Conclusions There are CD68+ monocytes and macrophages in the superficial dermis, forming a relatively dense network of defense. Such a distribution might indicate the clear polarity of the CD68+ monocytes/macrophages in the dermis, with their direction of defense towards the dermal-epidermal junction.更多还原