【摘要】 建立了HPLC-荧光法测定人血浆中西酞普兰的浓度,并进行了药物动力学研究。以盐酸普萘洛尔为内标,采用Dikma柱,以乙腈-0.03mol/L醋酸铵缓冲液(33∶67)为流动相,激发波长和发射波长分别为236和306nm。血浆中西酞普兰线性范围为1～75ng/ml,定量限为1ng/ml,方法回收率92.8%～98.8%。18名健康志愿者口服西酞普兰40mg后,血浆中的Cmax为(54.54±7.83)ng/ml,Tmax为(3.06±0.42)h,AUC0～132为(2351.1±389.9)ng·ml-1·h,AUC0～∞为(2636.5±444.9)ng·ml-1·h,t1/2为(40.45±8.68)h。更多还原

【Abstract】 A HPLC method with fluorescence detection for determination of citalopram in human plasma was established. Propranolol hydrochloride was used as the internal standard. A Dikma column was used with the mobile phase of acetonitrile-0.03mol/L ammonium acetate buffer solution(33∶67). The excitation wavelength and emission wavelength were 236 and 306nm, respectively. The calibration curve was linear in the concentration range of 1 - 75ng/ml. The low limit of quantification was 1ng/ml. The recovery was 92.8% - 98.8%. Eighteen volunteers orally taken 40mg of citalopram, the pharmacokinetics parameters were Cmax (54.54±7.83)ng/ml, Tmax (3.06±0.42)h, AUC0-132 (2351.1± 389.9)ng·ml-1·h, AUC0-∞(2636.5±444.9)ng·ml-1·h, t1/2 (40.45±8.68)h.更多还原