【摘要】 目的　探讨破骨样细胞(OLC)及其亚细胞成分对成骨细胞(OB)Cbfα1表达的影响。方法C57雌性小鼠,经尾静脉注射5 -FU后,取其脾脏细胞,在白介素(IL) 3、IL 6和粒细胞巨噬细胞集落刺激因子(GM CSF)、1α, 25 (OH)2D3的诱导下获得大量OLC。OLC在骨片上培养即获得骨片上的OLC。NaF、两种OLC、离心去除OLC的培养基及其亚细胞结构—细胞核、线粒体与OB共培养5d后,分别使用半定量RT PCR和免疫组织化学的方法检测OBCbfα1mRNA和蛋白质的表达活性。结果　NaF、两种OLC的细胞核、线粒体、细胞浆和离心去除OLC后的培养基均可使OBCbfα1mRNA的表达明显加强(均P<0. 05);NaF,两种OLC的细胞浆和离心去除OLC后的50%培养基均可显著增加OBCbfα1蛋白质水平的表达(均P<0. 05)。结论　OLC的亚细胞成分和离心去除OLC后的培养基均对OBCbfα1表达具有促进作用,同时Cbfα1的表达存在多水平的调节机制。更多还原

【Abstract】 Objective To investigate the effects of osteoclast-like cells (OLC) and their sub-cellular components on osteoblast (OB) Cbfα1 expression. Methods Spleen cells from C57 mice treated with 5-FU were induced with interleukin (IL)-3, IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), and 1α,25-(OH)_ 2D_ 3, and massive OLCs were obtained. These cells were also cultured on bone slides, yielding bOLCs. The OBs were co-cultured with two kinds of OLCs, NaF, the supernatant of OLC culture media, and the nuclei, mitochondria and cytoplasm of OLC for 5 days. The expression of OB Cbfα1 mRNA was evaluated by semi-quantity RT-PCR. OBs were observed by immunohistochemistry with Cbfα1 antibody. Results After the cultivation for 5 days , the Cbfα1mRNA expression of OB was significantly increased by NaF, the supernatant of two kinds of OLCs culture media, the nuclei, mitochondria and cytoplasm of OLCs (all P<0.05). By immunohistochemistry, Cbfα1 expression of OB incubated with the two kinds of OLCs cytoplasm and 50% supernatant of OLC culture media showed significant increment (all P<0.05). Conclusion The sub-cellular components of OLC and the supernatant of OLC culture media show a promoting effect on OB Cbfα1 expression. Cbfα1 expression is regulated at multiple levels in osteoblasts.更多还原