【摘要】 目的探讨自体骨骼肌肌源细胞(MDC)移植在压力性尿失禁治疗中的价值。方法采用差速贴壁法分离、纯化培养6只雌性SD大鼠的MDC,用携带Lac-Z基因的腺病毒载体转染MDC,转染成功后将MDC 5×106注射于自体膀胱颈周围,5、15 d处死大鼠切取膀胱及后尿道,组织学检查及X-gal染色观察注射组织形态学改善及MDC存活情况。结果5、15 d注射部位组织未见明显炎症改变及炎性细胞浸润,局部细胞层数增多。X-gal染色显示各时间点大量细胞被蓝染,提示移植细胞在体内成活。结论MDC自体膀胱尿道周围注射可长期存活,自体MDC移植有可能成为压力性尿失禁的一种有效的治疗方法。更多还原

【Abstract】 Objective To explore the value of muscle derived cell autologous transplantation in the treatment of stress urinary incontinence.Methods The skeletal muscle derived cells(MDC) from 6 female SD rats were isolated and purified by replate technique,and then the cells were transduced with adenovirus carrying Lac-Z gene.About 5×10~6 of MDCs were injected into the periphery of the bladder necks of the 6 autologous animals.The tissues were harvested 5 and 15 d after treatment,and histological examination and X-gal staining were performed.Results At 5 and 15 d after autologous MDC transplantation,histological examination revealed no apparent sign of inflammation and inflammatory cell invasion,but there were more layers of cells locally.X-gal staining showed a large number of cells expressing Lac-Z gene,which meant that the cells were alive.Conclusions The MDC autologous transplantation can survive permanently,which indicates that autologous muscle stem cells injection may be an effective treatment of stress urinary incontinence.更多还原