【摘要】 目的研究运动对高糖高脂饮食诱导的胰岛素抵抗大鼠脂肪细胞葡萄糖转运蛋白4(GLUT4)的影响。方法将实验大鼠随机分为正常组、模型组和运动组,每组各10只。各组以基础饲料适应性喂养1周后,模型组和运动组改用高糖、高脂饲料喂养,第5周时运动组进行为期6周的游泳训练。各组于实验第1,5和11周检测大鼠的体重、血甘油三酯、血胆固醇、空腹血糖和血浆胰岛素水平,计算胰岛素敏感指数。采用Westernblot法检测大鼠脂肪细胞内、外膜GLUT4含量。结果模型组大鼠与正常组相比,脂肪细胞内、外膜GLUT4含量均显著降低,差异均有统计学意义(P<0.05)。与模型组相比,经过6周游泳训练的运动组大鼠脂肪细胞内膜GLUT4含量无明显变化,而细胞外膜GLUT4含量显著增加,差异有统计学意义(P<0.05)。结论运动可提高胰岛素抵抗大鼠脂肪细胞外膜GLUT4含量,促进葡萄糖的摄取和利用。更多还原

【Abstract】 Objective To investigate the effect of exercise on glucose transporter 4 （GLUT4） in adipocyte of rats with insulin resistance induced by diets. Methods Experimental rats were randomized into three groups: a control group （n=10）, a model group （n=10） and an exercise group （n=10）. The model and exercise groups were fed with the diets enriched with sucrose （20%,w/w）, lard （10%, w/w）, cholesterol （2.5%, w/w） and cholic acid （1%, w/w） to induce insulin resistance. Exercise group was subject to swim for 6 weeks. After the plasma membrane and the intracellular membrane of adipocyte were prepared and separated, GLUT4 protein was detected by Western blot analysis. The body weight, serum triglyceride and cholesterol, fasting plasma glucose and serum insulin concentration were detected at the predetermined time points. And the insulin sensitivity index was calculated. Results Western blot analysis showed that intracellular membrane GLUT4 protein in adipocyte of the rats in the model group decreased by 38.72% （P<0.05） and plasma membrane GLUT4 protein decreased by 21.91% （P<0.05） as compared with those of the control group rats. After 6 weeks of exercise training, intracellular membrane GLUT4 protein of the rats of the exercise group did not change and plasma membrane GLUT4 protein increased 14.01% ^P<0.05 as compared with that of the model group rats. Conclusion Exercise training can increase the amount of GLUT4 protein in adipocyte of rats with insulin resistance and ameliorate translocation of GLUT4, enhancing the ingestion and utilization of glucose.更多还原