【摘要】 目的利用蛋白质组学技术识别结晶型硫化镍(NiS)诱发人支气管上皮细胞恶性转化相关蛋白的差异表达,探讨镍致癌的分子机制。方法应用二维凝胶电泳与相应软件分析结晶型NiS诱发细胞恶性转化后蛋白质组变化,基质辅助激光解吸电离-飞行时间-质谱(MALDITOFMS)结合数据库检索鉴定恶性转化相关蛋白。结果获得分辨率和重复性较好的二维凝胶电泳图谱,在相对分子质量14400～94000、等电点3～10范围内分离出约700个不同蛋白质点,对差异表达明显的等电点约6,相对分子质量约25000的蛋白质点进行质谱分析,鉴定出等电点为5.66,相对分子质量为21890的蛋白硫氧还原蛋白过氧化物酶2(Peroxiredoxin2,PDX2),并发现该蛋白在恶性转化细胞中高表达。结论蛋白PDX2可能是参与结晶型NiS诱发人支气管上皮细胞恶性转化过程的重要蛋白分子,与细胞恶性转化有关。更多还原

【Abstract】 Objective To provide evidence for illustrating the molecular mechanism of nickel carcinogenesis, and to identify the differential expression of protein in crystalline NiS-induced neoplastic transformation of human bronchial epithelial cell by proteomics technology.Methods Two dimensional electrophoresis(2-DE) and the ImageMaster 3.10 software were used to analyze the differential expression of protein,matrix-assisted laser desorption/inoization-time of flight mass spectrometry(MALDI-TOF-MS) combined with database search was applied to identify protein peroxiredoxin 2 (PDX2) related to malignant transformation.Results The good 2-DE pattern including resolution and reproducibility was obtained.Nearly 700 expressed proteins per 2-D gel were isolated with molecular weights(MW) ranging from 14 400 to 94 000 KD and pI 3-10.A protein PDX2 with MW 21 890 KD,pI 5.66, which was highly expressed in malignantly transformed cell, was identified using MALDI-TOF-MS.Conclusion PDX2 was involved in malignant transformation of human bronchial epithelial cell induced by crystalline nickel sulfide.更多还原