【摘要】 目的　探讨与支气管哮喘(简称哮喘)发作有关的外周血嗜酸粒细胞 (EOS)差异表达基因。方法　提取哮喘发作期患者外周血EOS总RNA为实验子(tester),治疗缓解期外周血EOS的总RNA为驱动子(driver);应用简单模块架构搜索工具(SMART)cDNA合成方法合成双链互补脱氧核糖核酸(cDNA),利用抑制消减杂交(SSH)技术筛选与哮喘发作有关的差异表达基因,进一步应用Southern印迹杂交验证差异表达基因。结果　共获得编码转化生长因子β活化激酶样蛋白(transformationgrowthfactorβactivatedkinaselike,TAK1L)、环磷酸鸟苷(cyclicguanosinemonophosate,cGMP)门控通道同源蛋白等 12个差异表达基因。结论　这些差异表达基因可能涉及EOS促炎症反应、细胞内信号传导、能量代谢及细胞凋亡等多种作用机制。进一步研究这些基因功能将帮助阐明EOS在哮喘发病更多还原

【Abstract】 Objective To study genes that differentially expressed in peripheral blood eosinophils of patient with asthmatic. Methods Total RNA extracted from eosinophils of patient at the time of exacerbation was taken as the tester and the total RNA from eosinophils of the same patient after improvement as driver. The ds cDNA was synthesized by SMART PCR cDNA Synthesis technology. The differentially expressed genes were obtained by suppression subtractive hybridization(SSH) technology and were further identified by Southern blot. Results Twelve differentially expressed genes including transformation growth factor β activated kinase like(TAK1L) protein, cGMP gated channel protein were obtained. Conclusion(Increased) expression of these genes were involved in the regulation of proinflammatory response, signal transduction, energy metabolism and cell apoptosis,which may help to clarify the molecular mechanism of eosinophils in asthma and may provide the theoretical base for finding out the new medicines towards eosinophils.更多还原