【摘要】 目的探讨白细胞介素-10(IL—10)基因对大鼠肝星状细胞(HSC)Ⅰ、Ⅳ型胶原表达及分泌的影响。方法将含有大鼠IL—10基因的腺病毒载体通过尾静脉转染四氯化碳肝损伤大鼠,用离体肝脏胶原酶灌注、消化以及密度梯度离心法分离HSC,应用逆转录聚合酶链反应法检测HSC的Ⅰ、Ⅳ型胶原mRNA 表达和酶联免疫吸附法检测HSC培养上清液中的Ⅰ、Ⅳ型胶原水平,用van Gieson染色检测胶原纤维在肝组织中的沉积情况。结果转染含IL—10基因腺病毒组HSC的Ⅰ、Ⅳ型胶原mRNA表达和分泌明显低于空载体组和空白对照组(P<0.05);van Gieson染色结果也显示胶原合成量明显少于空载体组和空白对照组。结论IL-10可以抑制HSC表达与分泌Ⅰ、Ⅳ型胶原。更多还原

【Abstract】 Objective To investigate the effect of interlukin-10 (IL-10) on expression and secretion of collagen I, IV in rat’s hepatic stellate cells (HSC) of livers injured by CCl4. Method The adenovirus vector encoded IL-10 gene was used to transfect rats with liver injury via the caudal veins. HSC were isolated and purified from the rat livers by collagenase IV perfusion and density gradient centrifugation with Nycodenz. The expression of collagen I, IV mRNA in HSC was detected by semi-quantitative RT-PCR method and the secretion of collagen I, IV in culture serum of HSC by ELISA method. The quantity of collagen was measured in the van Gieson stained histological liver preparations. Results The expression and secretion of collagen I, IV in the adenovirus vector encoding IL-10 gene group were significantly lower than those in the adenovirus vector without IL-10 gene group and the control group (P < 0.05). The quantity of collagen in the treatment group was lower than that in the control group. Conclusion IL-10 can inhibit collagen I, IV expression and secretion in rat HSC.更多还原