【摘要】 目的评价经导管动脉无水乙醇碘油乳剂化疗栓塞(TACE-EIOE)对肝细胞癌(HCC)预后的影响。方法经手术病理证实的HCC行TACE-EIOE治疗18例。用凋亡染色(TUNEL)法检测凋亡细胞,用免疫组织化学检测凋亡调节基因产物Bcl-2抗体、促凋亡基因产物Bax抗体、抑癌基因p53抗体、增殖细胞核抗原(PCNA)抗体和癌组织Ki-67抗体蛋白表达,观察生存率、病理及分子生物学改变。结果TACE-EIOE引起肿瘤完全坏死33.33%(6/18)、重度坏死44.44%(8/18)、中度坏死5.56%(1/18)、轻度坏死16.67%(3/18),细胞凋亡率(22.79±3.34)%,肿瘤包膜完整88.89%(16/18),显效66.67%(12/18),有效22.22%(4/18),稳定11.11%(2/18),Ki-67、PCNA、p53、Bcl-2、Bax蛋白表达率分别为(30.93±18.10)%、(41.16±11.83)%、(53.41±18.13)%、(6.32±2.10)%、(58.73±17.32)%。1、2、3年累计生存率,分别为83.33%、72.22%、66.67%。结论TACE-EIOE治疗HCC,安全有效。更多还原

【Abstract】 Objective To evaluate the effect of transcatheter arterial chemoembolization using ethanol and iodized-oil emulsion(TACE-EIOE) on prognosis of patients with hepatocellular carcinoma(HCC). Methods Eighteen patients with histologically-proven HCC were underwent TACE-EIOE. The extent of apoptosis was analyzed by terminal deoxynucleotidy transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) staining. The expressions of Bcl-2, Bax, p53, Ki-67 and PCNA proteins were detected by immunohistochemical method. Changes of these markers, tumor necrosis, encapsulation, volume, cumulative survival were analyzed. Results Complete tumor necrosis was 33.33%(6/18), severe tumor necrosis was 44.44%(8/18), moderate tumor necrosis was 5.56%(1/18), lesser tumor necrosis was 16.67%(3/18). Apoptosis rate was (22.79±3.34)%. Complete encapsulation was 88.89%(16/18). Evident volume-lessening was 66.67% (12/18), partial volume-lessening was 22.22%(4/18),and stable volume was 11.11%(2/18). Ki-67,PCNA, p53, Bcl-2, and Bax were (30.93±18.10)%, (41.16±11.83)%, (53.41±18.13)%, (6.32±2.10)%, and(58.73±17.32)%, respectively. The cumulative 1-, 2-,and 3-year survival rates were 83.33%,72.22%,and 66.67% for patients, respectively. Conclusions The preoperative TACE-EIOE is safe, it might benefit patients with HCC.更多还原