【摘要】 目的阐明电离辐射对小鼠EL-4淋巴瘤细胞P21蛋白表达的影响。方法采用免疫细胞化学方法和流式细胞术检测X射线照射对P21蛋白表达的时程和量效变化的影响。结果时程实验结果显示,离体培养的EL-4细胞经4.0Gy X射线照射后,P21蛋白表达在2～72h(免疫细胞化学方法检测)和8～72h(流式细胞术检测)明显增高(分别为P<0.01、P<0.001)。量效实验结果显示,X射线照射后24h,P21蛋白表达在0.5～6.0Gy(免疫细胞化学方法检测)和1.0～4.0Gy(流式细胞术检测)明显增高(分别为P<0.05、P<0.01、P<0.001)。结论X射线能诱导P21蛋白表达增高,在一定范围内存在时间和剂量依赖关系。更多还原

【Abstract】 Objective To explore the effects of ionizing radiation on the expression of P21 protein. Methods Immunocytochemistry and flow cytometry were used to measure the changes in the expression of P21 protein in EL-4 cells along with the increment of time and radiation doses. Results After 4.0 Gy X-rays irradiation, marked elevation of P21 protein level was found in EL-4 cells at 2-72 h with immunocytochemical method and at 8-72 h with flow cytometry （P<0.01 or P<0.001, respectively）. On the other hand, it was observed that the level of P21 protein increased evidently with the delivery of 0.5₆.0 Gy X-rays by using immunocytochemical method and with the delivery of 1.0₄.0 Gy X-rays by using flow cytometry in EL-4 cells at 24 h after irradiation. Conclusion The P21 protein expression could be increased by X-irradiation in time-and dose-dependent manners.;更多还原