【摘要】 目的筛选在小鼠精子发生过程中受电离辐射影响表达发生改变的印记基因。方法选择雄性BALBc小鼠8只,分为实验组和对照组X射线全身照射0.1Gyd,连续13d,建立辐射干扰小鼠精子发生模型。提取实验组和对照组睾丸RNA,采用上海生物芯片中心小鼠寡核苷酸基因表达谱芯片筛选表达发生改变的印记基因,对感兴趣的基因经半定量RTPCR验证。结果12个表达发生改变的印记基因,6个上调,6个下调,其中Igf2、Peg3基因Ratio值分别为3.859和0.397,经半定量RTPCR验证与芯片结果相符。结论X射线全身照射可导致小鼠睾丸部分印记基因表达发生改变。更多还原

【Abstract】 Objective To screen the imprinted gene with changed expression level by ionizing radiation in mouse spermatogenesis. Methods A mouse model, whose spermatogenesis was interfered by irradiation, was founded.A total of 8 male BALB/c mice were chosen and subjected to daily whole-body irradiation of 0^1 Gy X-rays for 13 days.Testis RNA from irradiated and control mice was extracted,the imprinted genes expressing variably were screened with mouse oligo gene expression chip supplied by Shanghai Biochip Center and the interested genes were verified by semiquantitative RT-PCR. Results Altogether 12 imprinted genes were found,6 of them were up-regulated,and another 6 down-regulated,in which the ratio scores of Igf2 and Peg3 were 3^859 and 0^397,respectively.The results confirmed by semiquantitative RT-PCR were consistent with those of gene chip. Conclusion The expression of some imprinted genes in mouse testis might be changed by chronic X-ray whole body irradiation.更多还原