【摘要】 目的研究含金属硫蛋白(metallothionein,MT)蛋奶粉对小鼠电离辐射损伤的防护作用。方法受试小鼠连续灌胃含MT蛋奶粉14d后给予2.5GyX射线照射,24h后处死小鼠,检测其外周血白细胞数、淋巴细胞增殖率、股骨骨髓细胞DNA含量、胸腺细胞周期进程以及血液、肝脏和肾脏丙二醛(MDA)含量和抗氧化酶(SOD、CAT和GSHPx)活性。结果受照射小鼠给予含MT蛋奶粉后,外周血白细胞数、淋巴细胞增殖率、股骨骨髓细胞DNA含量和肝脏、肾脏、血浆SOD、CAT和GSHPx活性明显高于辐照对照组,而血液、肝脏和肾脏MDA含量明显降低。含MT蛋奶粉加照射组明显减轻了胸腺细胞G1期阻滞,促进其DNA合成。结论含MT蛋奶粉对小鼠辐射损伤具有防护作用。更多还原

【Abstract】 Objective To explore the protective effect of egg-milk with metallothionein (MT) on the mice injured by X-irradiation. Methods After fed intragastrically with egg-milk plus MT for 14 d, mice were irradiated with 2.5 Gy X-rays. Twenty-four hours after the mice were irradiated, the number of WBC in peripheral blood, lymphocyte proliferation rate, DNA content of marrow cells, thymocyte cycle progression, malondialdehyde (MDA) level and the activities of SOD, CAT, GSH-Px in liver, serum and kidney were measured. Results In irradiated mice fed with egg-milk plus MT, the WBC number, lymphocyte proliferation rate, DNA content of marrow cells, the activities of SOD, CAT and GSH-Px in liver, kidney and serum were all significantly higher than those in the irradiation alone group. Besides in the former group the MDA level decreased significantly, the G-1 phase arrest in thymocytes was weakened and DNA synthesis was promoted. Conclusion The egg-milk with MT has protective effect on the mice injured by X-irradiation.;更多还原