【摘要】 目的:观察芦荟粗多糖对体外培养表皮细胞分泌生长因子(EGF,TGF-α,TGF-β1)、白细胞介素(IL-1β,IL-6,IL-8)及肿瘤坏死因子(TNF)的影响。方法:用不同剂量(75,150,300,600,1 200 mg.L-1)芦荟粗多糖作用于表皮细胞,采用ELISA和放射免疫法(RIA)测定细胞培养上清液中EGF,TGF-α,TGF-β1,IL-1β,IL-6,IL-8,TNF的水平,并以等体积的细胞培养液处理为对照组。结果:经芦荟粗多糖作用后,表皮细胞培养液中EGF,TGF-α,TGF-β1,IL-1β,IL-6,IL-8,TNF水平呈不同程度升高,其中EGF,TGF-α,IL-1β,IL-6,IL-8水平与对照组比较其差异具有显著性意义(P<0.05,P<0.01),且呈一定的量效关系。结论:芦荟粗多糖对表皮细胞分泌EGF,TGF-α,IL-1β,IL-6,IL-8具有促进作用。更多还原

【Abstract】 Objective: To study the effects of Aloe coarse polysaccharide on the levels of growth factors（EGF,TGF-α,TGF-β1） and interleukins（IL-1β,IL-6,IL-8） and tumor necrosis factor（TNF） in cultured keratinocytes.Method: The cultured keratinocytes were treated with Aloe coarse polysaccharide at concentrations of 75,150,300,600,1 200 mg·L^-1and the equal volume of media as control group.The levels of EGF,TGFα,TGF-β1,IL-1β,IL-6,IL-8 and TNF in the supernatants of cultured keratinocytes were assayed by enzyme-linked immunosorbent assay（ELISA） and radioimmunoassay（RIA）.Result: Compared with the control group,the levels of EGF,TGF-α,IL-1β,IL-6 and IL-8 were significantly increased by treatment with Aloe coarse polysaccharide（P<0.05,P<0.01） and in a dose dependent manner,and the levels of TGF-β1 and TNF were also increased but no statistical significance.Conclusion: Aloe coarse polysaccharide may promote keratinocytes to secrete EGF,TGF-α,IL-1β,IL-6 and IL-8.更多还原