【摘要】 目的:探讨线粒体在土贝母苷甲(TBMS1)诱导人宫颈癌HeLa细胞凋亡过程中的作用。方法:流式细胞仪测定线粒体膜电位(ΔΨm)、琼脂糖凝胶电泳分析DNA断裂片和蛋白质免疫印迹法检测细胞色素c(cyt c)的表达。结果:TBMS1引起人宫颈癌HeLa细胞凋亡,使得ΔΨm下降,释放入胞质的cyt c增加,并具有时间、剂量依赖性;TBMS1还可直接作用于分离的鼠肝脏线粒体,使之释放cyt c;TBMS1的这种作用部分能够被线粒体膜保护剂环孢菌素A(CsA)阻断。结论:线粒体途径是TBMS1诱导的HeLa细胞凋亡的途径之一。更多还原

【Abstract】 Objective: To investigate the role of mitochondria in TBMS1-medited apoptosis of human cervical carcinoma HeLa cell line.Method: Mitochondrial transmembrane potentia(Δψm) was assayed by flow cytometry.Apoptotic induction by TBMS1 was determined by gel electrophoresis of fragmented DNA.Cytochrome c(Cyt c) was detected by Western blotting.Result: The results showed that TBMS1 induced apoptosis in HeLa cells,that TBMS1 decreased Δψm and facilitated Cyt c release,and that TBMS1 induced apoptosis of HeLa cells dose-and time-dependently in accordance with increase of cytosolic Cyt c.TBMS1 had direct effect on isolated rat mitochondria which induced a time-and dose-dependent release of Cyt c from mitochondria.The results also showed that CsA protected partly HeLa cells from the effect of TBMS1.Conclusion: The mitochondrial apoptosis pathway has effects on TBMS1 induced human cervical carcinoma HeLa cell line apoptosis.更多还原