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滇姜花中姜花酮的含量测定
Determination of hedychenone in Hedychium yunnanense
【摘要】 目的:建立滇姜花Hedychium yunnanense的二萜类成分姜花酮(hedychenone)的含量分析方法。方法:C18柱,流动相乙腈-水(9∶1),柱温25℃,流速1.0 mL.min-1,检测波长235 nm。结果:hedychenone的线性范围5.92~29.6μg.mL-1(r=0.999 9)。平均加样回收率为98.9%。精密度RSD<2%(n=5)。结论:本方法简单、有效、可行,可用于滇姜花的质量评价。
【Abstract】 Objective: To establish an HPLC method for determination of hedychenone in Hedychium yunnanense.Method: C18 Chromatographic column was used,acetonitrile-water(9∶1) as mobile phase,at flow rate of 1.0 mL·min-1.The wavelength for detection was 235 nm.Result: The linear range of hedychenone was 5.92-29.6 μg·mL-1(r=0.999 9).The average recovery was 99.0%,RSD of precision was less than 2%.Conclusion: The method is simple,effective and feasible,and can be used to evaluate the quality of the herb.
- 【文献出处】 中国中药杂志 ,China Journal of Chinese Materia Medica , 编辑部邮箱 ,2005年20期
- 【分类号】R284.1;
- 【被引频次】3
- 【下载频次】196