【摘要】 目的:建立同时测定精制桂枝茯苓胶囊中3个有效成分——丹皮酚、苦杏仁苷、芍药苷含量的HPLC分析方法。方法:C18柱,流动相乙腈-水(含1‰磷酸),阶梯洗脱,流速1mL·min-1;MWD检测器。结果:苦杏仁苷线性范围为12.87-102.94μg·mL-1,r=0.9999;芍药苷线性范围为24.84-198.7μg·mL-1,r=0.9999;丹皮酚线性范围12.57-100.56μg·mL-1,r=0.9999。3个化合物的精密度与重复性RSD均<1.5%。结论:该法精密度高,重复性好,又可降低质量检验成本,可用于精制桂枝茯苓胶囊的质量控制。更多还原

【Abstract】 Objective: To establish an HPLC method for the simultaneous determination of three major bioactive components in Jingzhi Guizhi Fuling capsules namely laetrile, paeoniflorin and paeonol. Method: A LiChrospher C18 column(4.6 mm×250 mm,5 μm) was used. The chrornatography was carried out with a stepwise gradient programming. The mobile phase was acetonitrile-water (containing 0.1% phosphorous acid ) and the flow rate was 1.0 mL·min1. Result: The linear range of laetrile was 12.87-102.94 μg·mL-1, r=0.999 9, paeoniflorin 24.84-198.7 (μg·mL-1,r = 0.9999 and paeonol 12.57-100.56 μg·mL-1, r = 0.999 9. The method is accurate with variation less than 1.5 % and recovery more than 95 % . Conclusion: The method was successfully applied to analyze three major bioactive components in Jingzhi Guizhi Fuling capsules.更多还原