【摘要】 目的:建立并评价可供直接观察药物治疗作用的大鼠急性不全梗阻性胆管炎的实验模型。方法:选用健康Wistar大鼠行胆管插管,并于近肝门处借由硅胶管的支撑作用适度结扎,造成胆管的不全梗阻,然后经导管逆行注入浓度为1×10 9cFu/mL的大肠杆菌。术后72h取肝脏行病理学检查,并测定肝功能、血清细胞因子及血浆内毒素水平。结果:与假手术组大鼠相比,模型组大鼠血中ALT、AST、TBIL水平显著升高,血清TNF、IL - 1、IL - 6和血浆ET水平显著增加,病理学检查亦证实肝胆结构病理改变明显。结论:建立的急性不全梗阻性胆管炎实验模型,方法可靠,术后可直接予利胆治疗而无须再次手术解梗,简便经济更多还原

【Abstract】 Objective To establish and evaluate acute cholangitis rat model,so as to provide the foundation for the study of therapeutic effects of herbs Methods Healthy Wistar rats were selected and the bile duct near the hepatic porta was intubated retrogradely,then the bile duct was ligated properly near porta hepatis to cause partial obstruction of the duct,followed by intracholedochal injection of 1×10~9 cFu/ml E.coli.After 72 hours,liver tissue was examined by microscopy.Liver function tests and the levels of cytokines and endotoxin were also measured. Results The liver function parameters and the levels of cytokines and endotoxin were aggravated remarkably.The pathologic changes of the liver were also obvious. Conclusion The method of making acute cholangitis rat model by partly ligating the bile duct and intraledochal injection of E.coli is reliable,convenient and economic,thus providing the fundation for the study of therapeutic effects of herbs.更多还原