【摘要】 目的:观察N-Ras/pERK1/2在多发性骨髓瘤(MultipleMyeloma,MM)中的表达及临床意义,探讨N-Ras/MAPK信号通路与多发性骨髓瘤发生、发展之间的相互关系。方法:选择多发性骨髓瘤患者28例,应用Westernblot印记杂交检测N-Ras、pERK1/2蛋白表达水平,分析两者表达的相互关系。应用聚合酶链反应(PCR)/限制性片段长度多肽性分析法(RFLP),单链构象多肽性分析法(SSCP)和DNA测序方法对多发性骨髓瘤患者进行N-Ras基因12位点突变分析。结果:多发性骨髓瘤N-Ras、pERK1/2的表达水平(N-Ras:0.56±0.19;pERK1/2:0.39±0.21)显著高于正常人末梢血淋巴细胞(N-Ras:0.13±0.12;pERK1/2:0.10±0.14()P<0.01);28例多发性骨髓瘤患者中未发现N-Ras基因12位点突变;相关性分析结果表明多发性骨髓瘤N-Ras与pERK1/2的表达具有密切相关性(r=5.1326,P<0.01)。结论:非突变型N-ras基因产物介导的ERK信号传导通路参与了多发性骨髓瘤的发生、发展过程。更多还原

【Abstract】 Objective: To investigate the expression and its clinical significance of mutated/non-mutated (Codon 12) N- Ras and pERK1/2 (Extracellular signal- regulated kinase), in order to clarify therelationship between expression of Ras and ERK in Multiple Myeloma (MM). Methods: Western blotanalysis was used to detect the expression level of N- Ras and pERK1/2, and mutation analysis at N-ras codon 12 was finished using the method of PCR/RFLP, PCR/SSCP and DNA sequencing analysis, inthe clinical samples from 28 diagnosed MM patients. Results: The expression level of N- Ras andpERK1/2 (N- Ras: 0.56±0.19; pERK1/2: 0.39±0.21) was significant higher in the clinical samples suf-fering from multiple myeloma than that in the group of peripheral blood lymphocytes of volunteer (N-Ras:0.13±0.12; pERK1/2: 0.10±0.14, P<0.01). There was no mutation at N- ras codon 12 in all of patientssuffering from MM. Close relationship was found between the expression level of non- mutated N- Rasand pERK1/2 in Multiple Myeloma (r=5.1326, P<0.01). Conclusions: There is a close relationship be-tween the expression of N- Ras and the pERK1/2 in Multiple Myeloma. Ras/ERK signaling pathway,seldom affected by N- ras (Codon 12) mutation, may play some roles in the development of humanMultiple Myeloma.更多还原