【摘要】 目的:探讨合成紫花茄皂苷对BEL-7402细胞的增殖抑制及凋亡诱导作用。方法:采用酸性磷酸酶(APA)法、吖啶橙荧光染色法和流式细胞术检测合成紫花茄皂苷对人肝癌BEL-7402细胞增殖的影响以及诱导细胞凋亡的作用。利用蛋白印迹法检测药物作用后凋亡相关蛋白PARP的表达水平。结果:合成紫花茄皂苷对人肝癌BEL-7402细胞有明显的增殖抑制作用,其72h的IC50为4.2μg/ml。荧光显微镜下细胞呈现典型的凋亡形态。流式细胞术分析发现,细胞经皂苷作用6h、12h和24h后,凋亡率显著增加。蛋白印迹检测结果显示,合成紫花茄皂苷作用后肿瘤细胞内的PARP蛋白被剪切,Bcl-2蛋白的表达量下调。结论:合成紫花茄皂苷对人肝癌细胞的增殖抑制呈浓度相关性,具有诱导肿瘤细胞凋亡的作用,且这一作用可能与Bcl-2表达下调有关。更多还原

【Abstract】 Objective: To investigate the anti-proliferation effect and apoptosis-inducing activity of synthetic saponin in Solanum indicum L. on human hepatocarcinoma BEL-7402 cells. Methods: The acid phosphatase assay (APA), Acridine orange(AO) fluorescent staining and flow cytometry were used to evaluate the anti-proliferation and apoptosis-inducing effects of the saponin on BEL-7402. Expression levels of poly (ADR-ribose) polymerase (parp), an apoptosis related protein, was measured by Western Blot. Results: The solanum saponin (indiaside) significantly inhibited proliferation of BEL-7402 cells with IC50 value of 4.2μg/ml-1 for 72 hours. Typical apoptotic morphological features were observed by fluorescent microscopy on the saponin treated cells. Apparent enhancement in apoptosis rate was detected using flow cytometry, after BEL-7402 cells had been exposed to the saponin for 6 hours, 12 hours and 24 hours, respectively. The Western Blot analysis showed that PARP was proteolysized and Bcl-2 protein levels was decreased by saponin treatment. Conclusion: The saponin has dose-dependent inhibition effects on proliferation of human hepatocarcinoma cells, and can induce apoptosis, probably by decreasing Bcl-2 expression.更多还原