【摘要】 目的:研究明黄膏质量标准。方法:采用薄层色谱(TLC)法对明黄膏中的主要成分大黄、黄连、苦参进行定性鉴别;高效液相色谱(HPLC)法测定明黄膏中大黄酚的含量。结果:TLC色谱中均能明显地检出大黄、黄连、苦参;HPLC法测得本品中大黄酚的含量为0.267~0.308 mg·g-1;在10.02~100.16 mg·L-1的范围内,溶液的浓度与峰面积呈良好线性关系,r=0.999 9;加样平均回收率为98.48% (n=6),RSD为1.20%。结论:本品定性、定量方法简便、准确,专属性强,质量标准能够控制该制剂的内在质量。更多还原

【Abstract】 OBJECTIVE To study the quality standard of Minghuanggao gel. METHODS Radix et Rhizoma Rhei, Rhizoma Coptidis and Radix Sophorae Flavescentis in Minghuanggao gel were identified by TLC. The content of chrysophanol in this preparation was determined by HPLC. RESULTS Radix and Rhizoma Rhei, Rhizoma Coptidis and Radix Sophorae Flavescentis in Minghuang-gao gel could be detected obviously by TLC. The content of chrysophanol in this gel was 0.267 - 0.308 mg·g -1 . The linear ranges were 10.02 - 100.16 mg·L -1 with good positive correlation. The average recovery of chrysophanol was 98.48% ( n =6),RSD= 1.20% . CONCLUSION TLC is a simple, method HPLC is accurate and reliable method. The quality standard can be used for quality control of this product.更多还原