【摘要】 目的 建立反相高效液相色谱法对对香豆酸的药动学进行研究。方法 血浆样品用甲醇沉淀蛋白,采用Diamonsil C18色 谱柱(4．6 mm×200 mm,5μm),流动相为乙腈-1％冰醋酸(体积比为21:79),流速1．0 mL·min_-1,检测波长为322 nm,内标为橙皮 苷。结果 对香豆酸在0．085～10．6 mg·L-1(r=0．999 3)时其浓度与对香豆酸和橙皮苷峰面积比呈良好的线性关系,定量限 为0．085 mg·L-1。大鼠腹腔注射对香豆酸溶液后,对香豆酸的药动学行为符合二室模型,Ka为(0．38±0．09)min-1,t1／2(Ka)为 (1．85±0．20)min,t1／2(a)为(8．9±0．9)min,t1／2(β)为(34±3)min,k21为(0．045±0．008)min-1,k10为(0．040±0．009)min-1,k12为 (0．024±0．003)min-1,AUC为(130±14)mg·min·L-1,tmax和ρmax实测值分别为(6．0±0．8)min和(3．5±0．4)mg·L-1。结论 该 方法灵敏、准确,适合于对香豆酸的药动学研究。更多还原

【Abstract】 OBJECTIVE To develop a sensitive and specific HPLC method for the pharmacokinetics of p-coumaric acid in rats.METHODS The plasma samples were purified after the precipitation of protein with methanol. The separation was performed on a Diamonsil C18 column with the mobile phase consisting of acetonitrile-1% acetic acid (21:79) at a flow rate of 1.0 mL·min-1.The ultraviolet detector was operated at 322 nm. The internal standard was hesperidin. RESULTS The linear range was 0.085-10.6mg·L-1(r=0.9993) . The detection limit of this method was 0.085 mg·L-1. The concentration-time curve of p-coumaric acid in rat after ip administration was described by a two-compartment open model.The pharmacokinetic parameters of p-coumaric acid were as follows: Ka(0.38±0.09) min1-, t1/2(Ka) (1.85±0.20)min,t1/2(a) (8.9±0.9)min, t1/2(β) (34±3)min, K21(0.045±0.008)min-1,k10(0.040±0.009)min-1,k12(0.024± 0.003)min-1,AUC(130±14)mg·min·L-1,tmax(6.0±0.8) min and ρmax(3.5±0.4) mg·L-1.CONCLUSION The method is simple, rapid and applicable with a good reproducibility.更多还原