【摘要】 目的对大鼠血浆补阳还五汤中黄芪甲苷的含量进行了测定,并进行了药动学研究。方法采用反相高效液相色谱法测定,其条件为色谱柱:C₁₈柱（4.6mm×250mm,5μm）;检测波长:203nm;流动相:乙腈-水（35:65）,流速:1mL·min^-1;柱压:（15.17±0.4137）MPa;温度:45℃。药动学参数采用3P87软件处理。结果血浆样品中黄芪甲苷线性范围为2.4～12μg,r=0.9996,回收率为100.2%,RSD为1.100%。黄芪甲苷药动学参数:复方:t_1/2α为1.392h;t_1/2β为14.24h;K₁₂为0.1624h^-1;K₂₁为0.3045h^-1;K₁₀为0.07958h^-1;总苷:t_1/2α为2.641h;t_1/2β为37.12h;K₁₂为0.1283h^-1;K₂₁为0.1071h^-1;K₁₀为0.04574h^-1。结论该法快速、简便,准确。补阳还五汤复方及总苷中黄芪甲苷药动学为二室模型。中药复方成分组合对药动学参数有一定影响。更多还原

【Abstract】 OBJECTIVE To develop a HPLC method for the determination of astragaloside Ⅳ in rat plasma. And to investigate astragaloside Ⅳ pharmacokineties in rat. METHODS The separation was performed on a C₁₈ column （4.6 mm×250 mm, 5 μm）. The detection wavelength was at 203 nm. A solution of acetonitrile-water（35: 65）was used as the mobile phase with the flow rate of 1 mL·min^-1. The temperature was 45℃. The pharmacokinetic parameters were evaluated with 3P87. RESULTS The calibration curve was linear in the range from 2.4 to 12 μg （r=0.9996）. The average recovery was （100.2±1.103）% and RSD was 1.100%. The pharmacokinetic parameters of astragaloside Ⅳ was as following: t_1/2α was 1.392 h; t_1/2β was 14.24 h; K₁ was 0.1624 h^-1; K₂₁ was 0.3045 h^-1; K₁₀ was 0.07958 h^-1. The pharmacokinetic parameters of total glycoside were as following: t_1/2α was 2.641 h; t_1/2β was 37.12 h; K₁₂ was 0.1283 h^-1; K₂₁ was 0.1071 h^-1; K₁₀ was 0.04574 h^-1. CONCLUSION The method is rapid, simple anti easy to use for the determination of astragaloside Ⅳ in rat plasma after the treatment of Buyang Huanwu decoction. The pharmacokinetics of astragaloside Ⅳ in rat can be discribed by two compartmental model.更多还原