【摘要】 目的测定湖南产6种(含一新种)石杉属植物中石杉碱甲含量,为科学利用该植物提供可靠依据。方法于该省野外实地采集石杉属植物,以2%酒石酸溶液提取总碱,用改进的高效液相色谱法测定石杉碱甲的含量(外标法)。结果所采集6种石杉属植物中,石杉碱甲在蛇足石杉、狭叶石杉、长柄石杉、四川石杉、湖南石杉(拟新种)中均有分布,长柄石杉中含量最高,达0.0789%;本实验证明皱边石杉中不含石杉碱甲,先前两篇高含量报道应为鉴定之误。结论本测定方法简便、准确;在该属中4种形态极难区分的种之间,石杉碱甲含量具有分类学意义。更多还原

【Abstract】 OBJECTIVE To determine the content of huperzine A in six plants (including a novel species) of Genus Huperzia Bernh. from Hunan provinee. METHODS Plants were. colleeted in the wild field in Hunan province. Total alkaloids were extracted by 2% tactic acid, and were analyzed by an improved HPLC. The content of huperzine A was determined by external standards. RESULTS Six species from Huperzia Bernh. were gathered. Huperzine A was found in H. serrata, H. serrata var. intermedia, H. sutchueniana, H. hunaniana and especially in H. javanica, whose content reach up to 0.078 9%. The content of huperzine A in H. crispata was not observed. CONCLUSION The method is simple and aeeurate. The content of huperzine A is an aidant taxonomic character in four species that are hard to be morphologically differentiated from each other.更多还原