【摘要】 目的从红花中提取水溶性粗多糖,进一步纯化,得到均一的多糖CTP,并研究其组成和性质。方法粗多糖经反复冻融、Sevage法脱蛋白、超滤、柱色谱等方法分离纯化得到CTP。经比旋光度测定、HPLC等方法检验其均一性,结果CTP为均一组分,GC分析其单糖组成为Gle和Cal,摩尔比为6.08:1 结论CTP为中性杂多糖,相对分子质量4 000-5 000。更多还原

【Abstract】 OBJECTIVE To obtain a homogeneous polysaccharide CTP (a water-soluble polysaccharide from Carthamus tinctorius L.) and study its composition and charaterization. METHODS After the crude polysaccharide was freezed-thawed repeatedly, deprotinized Sevage method and fractionated by ultraflltration and gel chromatography, the water-soluble polysaccharide CTP was obtained. The homogeneity was proved by specific optical rotation measurement and HPLC method. RESULTS CTP was homogeneous and consisted of Glc and Gal in the molar ratio of 6.08: 1. CONCLUSION CTP is a neutral heteropolysacchride and its molecular weight is about 4 000 - 5 000.更多还原