【摘要】 以大田种植的2个半冬性甘蓝型油菜品种为供体材料,对游离小孢子培养的合适时期、加倍方法、再生苗的继代与移栽技术进行了研究。结果表明:小孢子培养的最佳时期为初花期前后2周左右,游离小孢子经过含有50mg/L或75mg/L秋水仙碱的NLN-16培养基直接处理48h效果最好,其产胚率和加倍效率均显著高于未加秋水仙碱的对照。在固体B5 培养基中加入4. 5mg/L多效唑并补充液体B5 -3培养基,再生苗可不需继代。在固体B5 培养基或者补充养分的液体B5 -3培养基中加入低浓度的0. 5mg/LNAA+0. 1mg/L6-BA,直接移栽到大田的再生苗成活率可以达到90%以上。更多还原

【Abstract】 The appropriate time of isolated microspore culture, efficiency of chromosome doubling with different colchicine concentrations and the methods for subculturing and transplanting regenerated plants were investigated, using two semi-winter type Brassica napus L varieties naturally planted in Wuhan. The result showed that the optimal time for isolating microspores was approximately 7 days before or after the first flower emergence. Yield and doubling efficiency were significantly higher than the control when the isolated microspores were treated by NLN-16 with the addition of 50mg/L or 75mg/L colchicine. Subculture of regenerated shoots was not necessary with the supplement of 4.5mg/L Paclobutrazol to solid medium B₅. With 0.5mg/L NAA+0.1mg/L 6-BA in solid B₅ medium or liquid B₅-3 medium, above 90% regenerated shoots directly transplanted in the field could survive.更多还原