节点文献
家兔全血基因组DNA提取方法
Methods of Genome DNA Extraction of Rabbits from Whole Blood
【摘要】 以肝素钠抗凝的皖系长毛兔全血为材料,以常规的酚氯仿抽提法为基础,采用改进的方法提取基因组DNA。结果表明,提取的基因组DNA纯度高,用琼脂糖凝胶电泳检测基因组DNA,主带清晰、无拖尾现象。
【Abstract】 The genome DNA of Wan line Angora rabbits were extracted from froz en whole blood, anticoagulated with heparin- sodium, by the improved phenol/chlo roform method .The results showed that the purity of genome DNA was high. Detec ted by agar sugar gel electrophoresis, the major bands of genome DNA were clear without the phenomenon of long tail.
【基金】 安徽省自然科学基金(03041201);扬州大学自然基金(NK0313101)。
- 【文献出处】 中国养兔 ,Chinese Journal of Rabbit Farming , 编辑部邮箱 ,2005年06期
- 【分类号】S829.1
- 【被引频次】18
- 【下载频次】319