【摘要】 目的:探讨部分变性高效液相色谱(DHPLC)在检测胃癌组织、血浆中基因突变中的应用。方法:应用DHPLC对39例胃癌组织及血清中p53基因突变进行检测,并测序验证。结果:本研究中p53基因的突变率为21%(8/39);其中肠型胃癌突变率40%(6/15)明显高于弥漫型胃癌8.33%(2/24)(P<0.01);发现既往未见报道突变3例。血清标本中50%(4/8)可扩增出目的片段,但均未检出突变。结论:DHPLC是一项较好的检测胃癌组织中基因突变的筛查方法,在血清中的应用仍需探讨。更多还原

【Abstract】 Objective: To explore the effectiveness of denaturing high-performance liquid chromatography(DHPLC) by detecting the mutation in cancer tissues and serums of patients with gastric cancer.Methods: The mutation of p53 gene was detected by DHPLC in the specimens of cancer tissues and serums from 39 patients with gastric cancer,and sequencing was performed. Results: The mutation rate of p53 gene in gastric cancer tissues was 21.00%(8 of 39 cases);the mutation rate in intestinal-type gastric cancer(40.00%,6 of 15 cases) was higher than that in diffuse-type gastric cancer (8.33%,2 of 24 cases,P<0.01).Three new mutations were found.No p53 mutation was found in the serums.Conclusion: DHPLC is a convenient method for detecting the mutation in gastric cancer.更多还原