【摘要】 目的:比较青、老年大鼠心肌缺血再灌注损伤后氧化和抗氧化损伤改变,探讨其作用机制。方法:W istar大鼠48只,随机分为青、老年大鼠实验组和正常对照组。实验组制作心肌缺血再灌注模型。采用硫代巴比妥酸法测定心肌细胞MDA含量,亚硝酸盐法检测心肌细胞内Mn-SOD活性。结果:老年对照组及实验组心肌细胞Mn-SOD活性显著低于青年对照组及实验组(P<0.01);老年实验组心肌细胞MDA含量显著高于其对照组(P<0.01)。结论:心肌缺血再灌注损伤与活性氧引起的氧化损伤以及抗氧化作用的减弱有关;由于老年大鼠心肌氧化和抗氧化能力的增龄变化,使得损伤改变更为明显。更多还原

【Abstract】 Objective: To compare the oxidant/antioxidant state in myocardial ischemia-reperfusion injury between young and aged rats,and to further discuss the effects of age and ischemia-reperfusion on myocardial injury and its mechanism.Methods:A total of 48 rats were divided equally into 4 groups(n=12):young control group(YCG),young model group(YMG),aged control group(ACG),and aged model group(AMG).The ischemia-reperfusion models were made.Quantities of malondialdehyde(MDA) and activities of superoxide dismutase(SOD) were determined with thiobarbituric acid reaction and nitriteform method,respectively.Results:The myocardial SOD activities in ACG were significantly lower than those in YCG(P<(0.01)).The activities of myocardial SOD in AMG was significantly lower than those in YMG(P<0.01).The myocardial MDA content in AMG was significantly higher than that in ACG.Conclusion:The myocardial ischemia-reperfusion injury was correlated with both the increased oxidant injury induced by oxide radicals and decreased antioxidant protective action.The myocardial injury induced by ischemia-reperfusion in the aged rats was distinct compared with the young rats because of the age-dependent oxide and antioxidant efficiency on myocardial tissues.更多还原