【摘要】 目的:建立以苯酚-硫酸比色法测定云南榧子中多糖含量的方法。方法:检测波长为490nm,狭缝宽度为2nm,记录纸范围为20nm/cm;采用高效毛细管电泳法分析不同产地的云南榧子。结果:无水葡萄糖检测量在0·0114mg~0·1482mg范围内与吸收度线性关系良好(r=0·9994),平均加样回收率为98·3%(RSD=2·31%,n=5);不同产地云南榧子高效毛细管电泳指纹图谱基本一致,且主要含2种多糖。结论:不同产地云南榧子中多糖含量及组成无明显差异。更多还原

【Abstract】 OBJECTIVE:To establish phenol-vitriolic colorimetry for the assaying of polysaccharides in Torreya yunnanensis.METHODS:Detection wavelength was 490nm,slit-width was 2nm and record paper range was 20nm/cm;Torreya yun_nanensis from different origins were analyzed by HPCE.RESULTS:The linear range of anhydrous dextrose was 0.0 114mg～0.1 482mg(r=0.9 994);The average recovery rate was 98.3%(RSD=2.31%,n=5).The HPCE fingerprints of Torreya yu_nnanensis from different origins were basically the same,and 2 kinds of polysaccharides were contained in whcih.CONCLUSI_ON:The contents and compositions of polysaccharides of Torreya yunnanensis from different origins were of no significant diff_erence.更多还原