【摘要】 目的:建立以高效液相色谱法测定野菊花中木犀草素含量的方法。方法:色谱柱为Kromasil C18,流动相为四氢呋喃-甲醇-1.5%醋酸(27∶4∶69),流速为1.0ml/min,检测波长为353nm,柱温为30℃。结果:木犀草素进样量在0.028μg~0.56μg范围内线性关系良好(r=1.0 000),平均回收率为99.9%(RSD=2.28%)。结论:本方法操作简便,结果可靠,可用于野菊花药材的质量控制。更多还原

【Abstract】 OBJECTIVE:To establish a HPLC method for content determination of luteolin wild chrysanthemum flower.METHODS:The separation was performed on a Kromasil C18 column,the mobile phase was composed of tetrahydrofuran-methanol-1.5%acetic acid(27∶4∶69),the flow rate was 1.0 ml/min,the ultraviolet wavelength was 353nm,the column temperature was 30℃.RESULTS:The calibration curve showed good linearity in the range of 0.028μg～0.56μg(r=1.0 000),the average recovery was 99.9%(RSD=2.28%).CONCLUSION:The method is simple,convenient and accurate and can be used for the quality control of wild chrysanthemum flower.更多还原