【摘要】 目的:建立茶色素注射液中没食子酸含量的测定方法。方法:采用反相高效液相色谱法,色谱柱为C18,流动相为乙酸-甲 醇-二甲基甲酰胺-水(2:8:30:160),检测波长为280nm,流速为0．5ml／min,柱温为20℃。结果:没食子酸检测浓度在8．4- 92．4μg／ml范围内线性关系良好(r=0．9 997),平均加样回收率为99．13％(RSD=1．55％)。结论:本方法特异性、灵敏度、精密 度、回收率高,重现性好,可为茶色素注射液质量标准的制订提供依据。更多还原

【Abstract】 OBJECTIVE: To develop a method for the determination of gallic acid in tea pigment injection. METHODS: RP HLPC method was adopted in which the separation of gallic acid was performed on C18 chromatographic column with a column temperature of 20℃; the mobile phase was composed of acetic acid - McOH - DMF - H2O(2 : 8 : 30 : 160) with a flow rate of 0.5ml/ mm and detection wavelength of 280nm .RESULTS: The linear range of gallic acid was 8.4-92.4μg/ ml( r = 0.9 997),the average recovery rate was 99.13% (RSD = 1.55%) .CONCLUSION: The method developed in the present study is of high specificity .sensitivity .precision,recovery and reproducibility and it can provide a basis for the formulation of quality control method for tea pigment injection.更多还原