【摘要】 目的:测定并比较复方丹参片与滴丸中丹酚酸B和丹参酮ⅡA的含量。方法:色谱柱为EclipseXDBC18,丹酚酸B、丹参酮ⅡA流动相分别为甲醇-0·2%磷酸溶液(40∶60)、甲醇-水(85∶15),流速为1·0ml/min,柱温为30℃,检测波长为280nm。结果:丹酚酸B和丹参酮ⅡA进样量均在0·1μg~2·0μg范围内与峰面积值呈良好线性关系(r丹酚酸B=0·9998、r丹参酮ⅡA=0·9999);复方丹参片中丹酚酸B、丹参酮ⅡA的平均回收率分别为100·7%(RSD=1·9%)、101·0%(RSD=1·5%),复方丹参滴丸中丹酚酸B、丹参酮ⅡA的平均回收率分别为98·6%(RSD=1·2%)、98·8%(RSD=1·4%)。结论:本方法准确、可靠、重现性好;复方丹参片中丹酚酸B和丹参酮ⅡA含量较高,复方丹参滴丸中则未定量检出上述2成分。更多还原

【Abstract】 OBJECTIVE:To determine and to compare the content of salvianolic acid B and tanshinoneⅡ A in compound danshen tablets and its drop pills.METHODS:The determination was performed on Eclipse XDB-C 18 with column temperature at30℃,the mobile phases were methanol-0.2%phosphoric acid solution(40∶60)and methanol-water(85∶15),respective_ ly with the flow rate at1.0ml/min and the detective wavelength at280nm.RESULTS:Good linear relations were obtained when the sample size of salvianolic acid B and tanshinoneⅡ A were within the range of0.1μg～2.0μg(r=0.9998for salvianol_ ic acid B and r=0.9999for tanshinoneⅡ A );The average recoveries of salvianolic acid B and tanshinoneⅡ A of compound danshen tablets were100.7%(RSD=1.9%)and101.0%(RSD=1.5%),respectively;And those for the compound danshen drop pills were98.6%(RSD=1.2%)and98.8%(RSD=1.4%),respectively.CONCLUSION:The method was accurate,reˉliable and reproducible.The contents of salvianolic acid B and tanshinoneⅡ A in compound tanshen tablets are higher,while no fixed quantity were detected in the above2components in compound danshen drop pills.更多还原