【摘要】 目的:研究褪黑素(MT)对谷氨酸所致脑组织氧化损伤模型小鼠的对抗作用。方法:将小鼠随机分为空白组、模型组、MT(高、低剂量)组,MT组预先经腹腔注射给药7d ;经侧脑室注射谷氨酸造模,测定各组小鼠脑组织中的丙二醛(MDA)、谷胱甘肽(GSH)含量及谷胱甘肽过氧化物酶(GSH -PX)活性。结果:模型组小鼠脑组织MDA含量显著升高(P<0 .01) ,GSH含量显著降低(P<0 .01) ;与模型组比较,MT组MDA含量降低,GSH含量升高,GSH -PX活性增强。结论:MT可通过抗氧化及增强抗氧化酶活性抑制谷氨酸的神经毒性,发挥抗衰老作用。更多还原

【Abstract】 OBJECTIVE:To study the counteraction of melatonin(MT)on the oxidative damage model of brain tissues of mice caused by glutamic acid.METHODS:The mice were randomly divided into blank group,model group and melatonin(MT)(high dosage and low dosage)group.Drugs were injected(by intraperitoneal injection)into the mice of the MT group in advance for7days.Each group were injected with glutamic acid through lateral ventricle to form models,levels of MDA and GSH and the activity of GSH-PX of each group were determined.RESULTS:There was a significant increase in the level of MDA(P<0.01)and a significant decrease in the level of GSH(P<0.01)in the brain tissues of the model group;compared with the model group,there was a reduction in the level of MDA,an increase in the level of GSH and stronger of the activity of GSH-PX in the MT group.CONCLUSION:Melatonin(MT)could inhibit the neurotoxicity of glutamate and exert its anti-aging effect by antiox-idation and increasing the activity of antioxidase.更多还原