【摘要】 目的 :观察不同剂量的纳洛酮对大鼠局灶性脑缺血再灌注损伤模型的影响 ,并探讨其保护作用机制。方法 :将大鼠随机分为假手术组、模型组、纳络酮 (低、中、高剂量 )组和阳性对照组 (尼莫地平 ) ,采用改良的Longa法建立大鼠局灶性脑缺血再灌注损伤模型。各组腹腔注射给药结束后对大鼠神经功能进行评分 ,测量其脑梗塞面积、超氧化物歧化酶 (SOD )活性及丙二醛 (MDA)含量 ,并用电子显微镜观察脑组织超微结构变化。结果 :造模后 ,大鼠神经行为评分值升高 ,梗塞面积扩大 ,血清SOD活性降低 ,MDA含量增加 ,脑组织病理结构和超微结构明显受损。应用不同剂量的纳洛酮后均能改善上述指标。结论 :纳洛酮对大鼠局灶性脑缺血再灌注损伤模型具有保护作用 ,机制可能与其抑制脂质过氧化反应有关。更多还原

【Abstract】 OBJECTIVE:To study the influence of different doses of naloxone on focal cerebral ischemia reperfusion injury in rats and to explore its mechanism.METHODS:The rats were randomly divided into the sham operation group,the model group,the naloxone group(high dosage,moderate dosage,and low dosage)and the positive control group.The modified Longa method was applied to establish the models of focal cerebral ischemia reperfusion in rats.Infarct size,neurological deficit scores,superoxide dismutase(SOD)activity,malondialdehyde(MDA)content in serum of the model rats were detected.The ultrastructures of the brain tissues were observed by electron microscopy.RESULTS:Ischemia reperfusion injury in model rats resulted in increased neurological deficit scores,larger infarct size,decreased activity of SOD in serum and increased content of MDA.The pathological change and ultrastructural change were evident.The above indices were improved in all the groups administered with different doses of naloxone.CONCLUSIONS:Naloxone has protective effects on rat brain injury caused by focal cerebral ischemia reperfusion,whose mechanism might be related to inhibition of lipid peroxidation.更多还原