【摘要】 目的:检测基质细胞衍生因子-1α(SDF-1α)及相应受体血小板源性因子受体-4(CXCR4)在冠状动脉疾病中的表达水平,探讨SDF-1α在冠状动脉疾病中的作用及其临床应用价值。方法:分别采用酶联免疫吸附法和流式细胞法(FCM)检测42例冠心病患者[13例急性心肌梗死(急性心肌梗死组),14例不稳定性心绞痛(不稳定性心绞痛组),15例稳定性心绞痛(稳定性心绞痛组)]及16例正常健康对照者(正常对照组)血浆SDF-1α和其相应受体CXCR4表达水平。同时,分离不稳定性心绞痛组患者外周血单个核细胞进行体外研究,用酶联免疫吸附法测定SDF-1α(终浓度,500ng/ml)干预前后单核细胞趋化蛋白-1(MCP-1),肿瘤坏死因子-α(TNF-α)和组织型金属蛋白酶抑制剂-1(TIMP-1)的表达水平。结果:SDF-1α表达在稳定性心绞痛组有升高趋势,但与正常对照组相比较,差异无显著性(P>0.05);在不稳定性心绞痛组和急性心肌梗死组SDF-1α表达明显降低(P<0.05)。其相应受体CXCR4在稳定性心绞痛组表达有升高趋势,但与正常对照组相比较,差异无显著性(P>0.05);而在不稳定性心绞痛组和急性心肌梗死组CXCR4表达明显升高(P<0.01)。同时,体外研究结果显示,肿瘤坏死因子-α和单核细胞趋化蛋白-1在SDF-1α干预后的表达较干预前明显降低(P<0.01);而组织型金属蛋白酶抑制剂-1?更多还原

【Abstract】 Objective:We determined the plasma levels of stromal cell-derived factor-1α( SDF-1α) and its corresponding receptor CXCR4 at cellular level in peripheral blood mononuclear cell from patients with coronary artery diseases, and investigated the potential role and clinical application of SDF-1 a in coronary artery diseases. Methods: We determined the plasma level of SDF-1 a and its corresponding receptor CXCR4 at the cellular level in peripheral blood mononuclear cell from 42 patients with coronary artery diseases (13 with acute myocardial infarction, 14 with unstable angina and 15 with stable angina) and 16 healthy controls with enzyme linked immunosorbent assay(ELISA) and flow cytometry. Simultaneously, we isolated peripheral blood mononuclear cell from patients with unstable angina, then determine the level of monocyte chemotactic protein-1 ( MCP-1 ) , tumor necrosis factor-α(TNF-α) and tissue inhibitor of metalloproteinase-1 ( TIMP-1 ) before and after SDF-1α(final concentration:500 ng/ml) stimulation with ELISA. Results:Compared with the healthy control subjects, the plasma level of SDF-la in patients with stable angina was high,but the difference was not significant(p >0. 05). The plasma level of SDF-1α in patients with unstable angina and acute myocardial infarction significantly decreased (p < 0. 05 ). Compared with the healthy controls, the expression of its corresponding receptor CXCR4 in patients with stable angina was higher, but not significant (p >0. 05). The expression of CXCR4 in patients with unstable angina and acute myocardial infarction was significantly increased (p <0. 01 ) . In the in vitro study, compared with before SDF-1α stim lation,the level of TNF-α and MCP-1 in after SDF-1α stimulation was significantly decreased (p <0. 01). TIMP-1 in after SDF-1α stimulation was markedly increased(p <0. 01) . Conclusion:SDF-1α probably suppress the expression of proinflammatory cytokines and promotes the expression of anti-inflammatory cytokines. It can play a role in plaque-stabilizing effects in coronary artery diseases and thus reducing the incidence of acute coronary artery events.更多还原