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健康人群口腔酵母菌的分离及菌种分布

Isolation and distribution of yeast species in oral cavity of healthy population

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【作者】 白丽; 申元英; 王晶; 郭利军; 王涛; 申丽洁; 陈朝明; 潘云华; 林学颜;

【Author】 BAI Li 1,2 ,SHEN Yuan ying 1,WANG Jing 1, et al (1.Department of Medical Microbiology & Immunology,Dali College,Dali 671000;2.Department of Immunology,Sun Yat sen Medical College,Sun Yat sen University,Guangzhou 510089,China)

【机构】 大理学院医学微生物学及免疫学教研室; 中山大学中山医学院免疫学教研室 云南大理671000; 中山大学中山医学院免疫学教研室; 广东广州510089; 云南大理671000; 广东广州510089;

【摘要】 目的 调查健康人群口腔酵母菌的分布。方法 将来源于健康人的口腔拭子接种于改良SDA培养基,37℃培养14 d,分到的酵母菌通过菌落形态、革兰染色作初步鉴定,芽管形成试验、厚膜孢子生成及假菌丝产生试验、YBC酵母鉴定卡作菌种鉴定。结果 酵母菌在健康人口腔中总分离率为8.78% ( 86 / 979) ,其中较多的为白色假丝酵母菌37株,占4 3.0 2 % ,近平滑假丝酵母菌和季也蒙假丝酵母菌各9株,各占10 .4 7% ,葡萄牙假丝酵母菌6株,占6 .98% ,其他2 5株。结论 健康人口腔中有多种条件致病酵母菌的寄生

【Abstract】 Objective To explore the distribution of oral Yeast species in healthy population.Methods The oral swabs from healthy population were inoculated in the modified Sabouraud Dextrose Agar(SDA) and they were incubated at 37 ℃ for 14 days.The primary identification of Yeast was based upon the colony characteristics and the cell morphology by Gram stain.Germ tube,pseudohyphae and chlamydospores tests and VETIK YBC card were used to differentiate the various species.Results The positive rate of Yeast in the oral cavity of healthy po pulation was 8 78% (86/979).Of them,37 strains were Candida albicans and the component ratio was 43 02%.9 strains of C.Parapsilosis and C.guilliermondii were got separately and the component ratios were 10 47%.6 strains of C.lusitaniae were gained and the component ratio was 6 98%.25 strains of them belongs the other of Yeast.Conclusion There were multiple conditioned pathogenic Yeasts in oral cavity of healthy population.

【关键词】 酵母菌; 口腔; 健康人群;
【Key words】 Yeast; Oral cavity; Healthy population;
【基金】 国家自然科学基金资助项目 (编号 :30 2 60 0 0 5)
  • 【文献出处】 中国微生态学杂志 ,Chinese Journal of Microecology , 编辑部邮箱 ,2005年02期
  • 【分类号】R780.1
  • 【被引频次】15
  • 【下载频次】293
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