【摘要】 研究了溶剂、pH对异硫氰酸酯吸收光谱的影响,并报道了测定辣根及芥末制品中异硫氰酸酯含量的一种新方法。异硫氰酸酯在正己烷溶剂中的λmax=249nm,εmax=1041L/mol·cm。在249nm处测定样品正己烷提取液的吸光度,根据标准工作曲线,计算出样品的异硫氰酸酯含量。该法简便省时,灵敏度高,并通过与哌啶法对比实验,证明该法的准确性。更多还原

【Abstract】 In this study, the effects of solvent and pH on the UV- spectra of isothiocyanates were investigated, and a new method for quantitative determination of the isothiocyanates in horseradish, mustard jam and the related products has been developed. In hexane, maximum absorbance of isothiocyanates appears at λ_ max=249 nm (ε_ max=1041 L/mol·cm). Absorbance of the extractions of samples is determined at 249 nm and the amount of the isothiocyanates is calculated according to the standard curve. The proposed method is sensitive and convenient, and compared with piperidine method. The results indicated that the proposed method is accurate.更多还原