【摘要】 以牛肾细胞系(MDBK)培养牛传染性鼻气管炎病毒(IBRV)Bartha Nu/67株,经超速离心纯化病毒,再经超声破碎处理后作为诊断抗原,建立了检测牛血清IBRV抗体的间接酶联免疫吸附试验。该ELISA的判定标准为:血清D490 nm值大于0.369的判为阳性,小于0.295的判为阴性,在0.295与0.369之间的为可疑。特异性和重复性试验结果表明,该方法特异性高、重复性好。与法国进口ELISA抗体诊断试剂盒比较,其符合率为96.3%;与中和试验比较,符合率为95.8%,且敏感性更高。应用该诊断方法调查了我国部分地区IBRV的感染情况,结果显示,这些地区的IBRV感染率为67.1%。更多还原

【Abstract】 An infectious bovine rhinotracheitis virus（IBRV） strain was cultured in Madin-Darby bovine kidney（MDBK） cells and purified by ultracentrifugation.The purified virus was disrupted by using an（ultrasonic） disintegrator.An indirect enzyme-linked immunosorbent assay（ELISA） was developed to detect specific antibodies to IBRV in serum with prepared diagnostic antigen.D_490nm values higher than 0.369 were determined as positive standard for the ELISA and lower than（0.295） as negative standard.The assay was confirmed to be specific and reproducible.96.3% agreement was obtained by comparing to the IBR ELISA kit from France,and 95.8% by comparing to the virus neutralization test（VNT）.The IBRV infection in some areas of China was investigated by the established ELISA technique,and an average infection rate of 67.1% in cattle was obtained.更多还原