【摘要】 以昆明白品系小鼠胎儿生殖嵴为材料,以不同的培养液分离培养胚胎生殖细胞(EGcells),发现小鼠胎儿肝细胞条件培养液的效果好于未添加白血病抑制因子(LIF)的基础培养液,而差于添加了1 000 IU/mL LIF的基础培养液。同时,试验对比了从不同胎龄胎儿分离培养原始生殖细胞(PGCs)的情况,鉴定了EG细胞的生物学特性;EG细胞经体外培养,可以分化为神经样细胞、上皮样细胞和简单类胚体。更多还原

【Abstract】 An experiment was performed to compare effect of different cell media on embryonic germ(EG) cells derived from primordial germ ridge of Kunming albinotic strain mouse.The result showed that mouse fetus liver cells condition medium,which can maintain survivance and proliferation of mouse EG cells,was better than the medium containing no cytokine,but worse than EG cell medium with 1000(IU/mL) of LIF.At the same time,different age of fetuses were compared on effect of isolating and culturing PGCs,and the mouse EG cells had been identified by morphology,AKP stain and differentiation in vitro.更多还原