【摘要】 用摇瓶培养法,对用作生产传染性腔上囊病基因工程亚单位疫苗的基因工程菌种BL21/pET28a VP2的表达条件进行了优化研究。其结果为:温度 37 ℃,诱导表达时间 3~7 h,用乳糖诱导时终浓度为20 mmol/L时即达到最高表达量;用IPTG诱导时终浓度为0.33 mmol/L时即达到最高表达量;乳糖的诱导效果优于IPTG。更多还原

【Abstract】 Expressing condition of gene engineering strain Escherichia coli BL21/pET28a-VP2, which could be used for production of the subunit vaccine against infectious bursal disease, was performed using the method of rocking bottle culture. The result showed that the inducing temperature should be 37℃, the inducing expression time should last 3 to 7 hours, and the inducer could be lactoseor isopropyl-β-D-thiogalactopyranosid(IPTG). The maximum expression capacity could be obtained when the concentration of lactose reached 20mmol/L, or the concentration of IPTG reached 33mmol/L.更多还原