【摘要】 用PHA和LPS诱导猪脾和淋巴结淋巴细胞,提取细胞总 RNA,利用 RT-PCR技术克隆猪IL-6的cDNA并进行序列测定与分析。测序结果及同源性分析表明,已扩增到 IL-6 基因,大小约为669 bp,含有1个639 bp的开放阅读框,编码212个氨基酸,其中前28个氨基酸为信号肽,且碱基序列在不同种属动物间差别较大。分组诱导表明,LPS效果更佳,PHA和 LPS联合诱导能使更多的IL-6转录表达。更多还原

【Abstract】 The porcine IL-6 gene had been amplified by RT-PCR after porcine lymphocytes from spleen and lymph node was stimulated by LPS and PHA. The results showed that the porcine IL-6 gene had been successfully cloned and its open reading frame included 639bp nucleotides encoding 212 amino acids, the first 28 amino acids were signal peptides. The nucleotide sequence of the porcine IL-6 gene was significantly different from that of other species. The effect of LPS on inducing lymphocytes was better than that of PHA and the combination of LPS with PHA exerts the best effect.更多还原