【摘要】 利用 F41 单因子血清 IgG 致敏绵羊红细胞,建立了定量产肠毒素性大肠埃希氏菌(ETEC)F41黏附素的反向间接血凝试验。经致敏的绵羊红细胞能被F41单因子血清特异性地抑制,且不与 ETEC K88、ETEC K99、ETEC 987P、致仔猪水肿病大肠埃希氏菌 O138、鼠伤寒沙门菌和猪链球菌2型菌液出现交叉反应,其敏感性比甘露糖抵抗血凝反应(MRHA)至少高 25 倍。结果表明,该反向间接血凝试验具有很高的特异性和敏感性,可用于生物制品中黏附素含量的定量。更多还原

【Abstract】 A reverse indirect hemagglutination test（RIHA）was developed by using the monospecific anti-F （41） IgG sensitizing sheep red cells to quantitate F （41） positive Escherichia coli. The sensitized sheep red cells could be inhibited by monospecific anti-F （41）,and no cross reaction was observed on K （88）-ETEC,K （99）-ETEC,987P-ETEC,SLTEC（O （138））,Salmonella typhimurium and Streptococcus suis type 2. The sensitivity of RIHA was at least 2⁵ higher than that of the mannose-resistant hemagglutination（MRHA）.The results demonstrated that the RIHA is specific and sensitive and can be used to determine F （41） antigen contents in vaccines.更多还原