【摘要】 克隆了猪生殖与呼吸综合征病毒(PRRSV)SC2株ORF5基因。测序分析表明SC2株属美洲型PRRSV。构建的ORF5基因疫苗经脂质体转染Marc 145细胞后,第26h检测到疫苗蛋白的特异性表达,第56h达到高峰。疫苗肌肉注射小鼠和仔猪后能迅速分布到各组织器官中,未发现核酸疫苗与宿主细胞染色体整合现象;且能使仔猪产生体液和细胞免疫应答,免疫期持续135d以上。结果表明,构建的PRRSVORF5基因疫苗具有良好的安全性和免疫原性。更多还原

【Abstract】 ORF5 cDNA cloning and sequencing analysis of porcine reproductive and respiratory syndromevirus (PRRSV) SC2 strain showed that the strain belonged to PRRSV American type. The constructed ORF5 gene vaccinewas transferred into Marc-145 cell line. Intense fluorescence could be observed at 26th hour post-transfection(PT) in the Marc-145 cells and the amount of fluorescence peaked at 56th hour PT. The ORF5 gene could be detected by PCR from tissues and organs of mice and pigs immunized with the ORF5 gene vaccineby intramuscular injection. The ORF5 gene was not integrated into the chromosomes of the animal. Humoral and cellular immuneresponses were obtained in pigs by intramuscular injectionand gene gun transferroutes and persisted for more than 135 days. The results indicated that the ORF5 gene vaccineis immunogenicand safety.更多还原