【摘要】 为深化富硒灵芝的基础研究,指导富硒灵芝的生产与应用,以不同含硒量的富硒灵芝子实体为试样,系统地研究了硒在富硒灵芝中的分布,结果表明:富硒灵芝子实体中81.71%～87.92%的硒是以有机硒形式存在,并主要与子实体中的蛋白结合(蛋白硒占总硒的56.0%～61.2%);水溶性蛋白和碱溶性蛋白是灵芝结合硒的主要蛋白,总结合硒量占蛋白硒的75.2%～79.2%。按照结合硒量的多少,硒在蛋白中的分布依次为:水溶性蛋白>碱溶性蛋白>醇溶性蛋白>盐溶性蛋白。此外,应用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)分离富硒灵芝子实体中的含硒蛋白组分,并采用氢化物原子荧光法(HGAFS)对蛋白或多肽条带按分子质量段切割进行硒含量测定,结果表明:在分辨出的13条蛋白条带中,分子质量在8.709～142.53kDa的蛋白或多肽均结合了不同程度的硒,但硒主要分布在分子质量不超过16kDa的蛋白或多肽中。更多还原

【Abstract】 The distribution of selenium in Se- enriched Ganoderma lucidum (Se- GL) was investigated using Se- GL of different Se content as tested samples in order to promote both the research and application work in Se- GL. The results showed that 81.71～87.92% of total Se in Se- GL were in the organic form, and mainly associated with the protein in GL. Se bound to the protein extracts accounted for 56.0%～61.2% of total Se. Moreover, 75.2%～79.5% of Se bound to protein was incorporated with water- soluble and alkaline- soluble protein extracts. The amount of Se existed in the different pro- tein fractions decreased in the order of water- soluble, alkaline- soluble, ethanol- soluble, and sodium chloride- soluble protein extracts. Additionally, selenoproteins in Se- GL were also separated using sodium dodecyl sulfate- polyacrylamide gel electrophoresis ( SDS- PAGE) and analyzed Se content using atomic fluorescence spectrophoteometry ( HGAFS). 13 protein or polypeptide bands with molecular weight from 8.709 kDa to 142.53 kDa were identified. They all combined dif- ferent amount of selenium. However, selenium was mainly distributed in the proteins or polypeptides with molecular weight of no more than 16 kDa.更多还原