【摘要】 目的:对选育出的脂肽生物表面活性剂高产菌株SD59的摇瓶发酵条件进行了研究。方法:通过正交实验和单因素实验等方法研究了各种营养和环境因素对菌株SD59发酵生产脂肽生物表面活性剂的影响。结果:优化的培养基组成(g/L):可溶性淀粉20.0,硫酸铵6.0,硫酸镁0.3,氯化钙0.08,酵母粉0.6,磷酸二氢钾2.0,磷酸氢二钠8.0,初始pH7.2;最佳培养条件为500mL三角瓶的装液量为200mL,接种量5.0%,培养温度36～38℃。结论:在此优化发酵条件下,脂肽表面活性剂的产量可达到1.2g/L。更多还原

【Abstract】 Objective:In this article,the shaking flask culture conditions of strain SD59 which had a high productive capacity of lipopeptide biosurfactant have been studied. Methods:The influences of the nutritional and environmental factors on the fermentation production are investigated through the orthogonal experiments and single-factor experiments. Results:The optimized composition of medium(g/L):soluble starch 20.0,(NH4)2SO4 6.0,Na2HPO4·12H2O 8.0,KH2PO4 2.0,MgSO4·7H2O 0.3,CaCl2·2H2O 0.08,yeast powder 0.6,pH 7.2. The optimal culture condition:500 mL flask containing 200 mL medium,5.0% seed culture,temperature 36～38 ℃. Conclusion:On such condition,the productivity of lipopeptide can reach above 1.2 g/L.更多还原