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以nit和抗药性为遗传标记研究稻瘟病菌的无性重组

Study on Asexual Recombination in Magnaporthe grisea by Using Nitrate Non-Utilizing (nit) Mutants and Fungicide-Resistance as Independent Genetic Markers

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【作者】 张传清周明国

【Author】 ZHANG Chuan-qing, ZHOU Ming-guo~* (Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;)

【机构】 南京农业大学农业部病虫监测与治理重点开放实验室南京农业大学农业部病虫监测与治理重点开放实验室 江苏南京210095江苏南京210095

【摘要】 采用硝酸盐利用缺陷突变体(Nitratenon utilizingmutants,nit)为遗传标记研究了稻瘟病菌对异稻瘟净的抗药性在无性重组过程中的遗传学。在DY23(nit1LR)×A73(nitM S)、A73(nitM S)×F42(nit1MR)和F42(nit1MR)×DY24(nitA LR)3个配对组合中都检测到了无性重组体,重组频率分别为7.31%、14.00%和8.63%。大多数无性重组体都有较好的生长、产分生孢子能力和致病力,表明无性重组体有较高的适合度。这些结果说明由菌丝融合导致的无性重组在稻瘟病菌对异稻瘟净的抗药性群体发展中可能起着重要的作用。

【Abstract】 Genetics of kitazin P resistance in asexual recombination of Magnaporthe griseawas studied by using nitrate non-utilizing (nit) mutants. Asexual recombinants were detected in all the three pairings, namely DY2-3(nit1-LR)×A7-3(nitM-S),A7-3(nitM-S)×F4-2(nit1-MR), and F4-2(nit1-MR)×DY2-4(nitA-LR). The frequencies of the asexual recombinations were 7.31%, 14.00% and 8.63%, respectively. Most of recombinants had satisfied growth, spore production and pathogenicity ability, indicating that asexual recombinants had high fitness. These results suggested that asexual recombination caused by hyphal fusion might play an important role in the development of fungicide resistant population in M. grisea.

  • 【文献出处】 中国水稻科学 ,Chinese Journal of Rice Science , 编辑部邮箱 ,2005年03期
  • 【分类号】S435.111
  • 【下载频次】89
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